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RNA from Trizol in 7 minutes:
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RNA Clean & Concentrator Kits | RCC-100
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready, DNA-free (R1013, R1014) RNA.
Format
RNA Clean & Concentrator-100
Highlights
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
- Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
- Maximum Recovery: Recover > 90% and elute in as little as 100 µl.
Format
RNA Clean & Concentrator-100
Highlights
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
- Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
- Maximum Recovery: Recover > 90% and elute in as little as 100 µl.
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RCC-5Sample Available
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RCC-25
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RCC-100
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96-Well
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MagBead
| Cat # | Name | Size | Price | Quantity |
|---|---|---|---|---|
| R1019 | RNA Clean & Concentrator-100 | 25 Preps | $220.50 |
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| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| W1001-10 | DNase/RNase-Free Water | 10 ml | $22.10 | |
| R1060-2-10 | RNA Prep Buffer | 10 ml | $21.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| R1003-3-6 | RNA Wash Buffer | 6 ml | $21.00 | |
| R1013-2-100 | RNA Binding Buffer | 100 ml | $149.40 | |
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge and Vacuum Manifold |
|---|---|
| Format | Spin-Column |
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 1 mg RNA (binding capacity), ≥ 100 µl (elution volume) |
Resources
FAQ
Yes. The kit efficiently recovers all types of nucleic acids.
Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.
If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.
Both treatments will remove DNA efficiently. For pre-extracted RNA, perform in-tube DNase treatment followed by RNA clean-up.
Reviews
"Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant."
Catherine A.
USAMRIID
"Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do."
Caroline S.
Cornell University
"The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns."
Agnes N.
Biofidal
Citations
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